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Growing cells to express proteins may seem just a small step on the way to achieving your research goals, but…
- Uncontrolled growth leads to high oxygen demands and finally oxygen depletion in plates and shake flasks.
- Conventional mineral salt media, despite the use of buffering chemicals, suffer from decreasing pH as acid is generated and ammonia removed by microbial metabolism.
- As a result, low biomass and product yields are achieved.
Even the best-designed growth media will underperform if the surrounding environment is less than optimal. Comparison and optimization becomes difficult or even impossible.
With EnPresso® media, key elements to support growth, control pH and regulate glucose availability are provided:
- Cells grow steadily to reach significantly higher densities and continue in linear growth for many hours.
- Proteins are expressed under optimal metabolic conditions thereby reducing the risk of incorrect folding and increasing the percentage of soluble protein.
- Parallel cultivation can be conducted in a reproducible way to identify optimal operation.
Enpresso® products are based on the patented EnBase® technology (EP09154440, EP2130906, EP11151003).
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